Fig. 6

CLUSTALW alignment of ACR3 proteins and predictions of the transmembrane topology of Ml_ACR3. Secondary structure predictions for Ml_ACR3 were generated from the primary amino acid sequence [Ml_NCTC2665_ACR3(1)] with TMHMM 2.0 (TMs in shaded boxes), SOSUI [transmembrane segments (TMs) in filled boxes], and TopPred2 (TMs in open boxes). TopPred2 and SOSUI predicted nine TMs, whereas TMHMM 2.0 predicted ten TMs. Each TM is connected with cytoplasmic (long dashed lines) and periplasmic loop (dotted lines). Residues indicated under the alignments are corresponding to Ml_ACR3(1) (red), Cg_ACR3(1) (black), Am_ACR3(2) (green). The conserved residue Cys119 is located in TM4 with all predictions