Table 2 Primers used in this study for the amplification of 16S rRNA and arsenic (As) resistance-related genes
From: Genetic analysis of arsenic metabolism in Micrococcus luteus BPB1, isolated from the Bengal basin
Primer name | Primer sequence (5′-3′)a | PCR product (bp) | Reference |
|---|---|---|---|
fD1 | GAGTTTGATCCTGGCTCAG | 16S rRNA (1500) | Weisburg et al. 1991 |
rP2 | ACGGCTACCTTGTTACGACTT | ||
darsC_F | ACSATYTAYCAYAAYCCG | ArsC (377) | Branco et al. 2008 |
darsC_R | TCGCCRTCYTCYTTSGWRAA | ||
LMWP_F | ATGACCACGAAGCGCCCTTC | PTPase-ArsC (430) | This study |
LMWP_R | TCAGTTCGCGGTGCCGGT | ||
dLMWP_F | GCTGTTCGTGTGCGTGAARAAYGGNGG | PTPase-ArsC (357) | This study |
dLMWP_R | CGATGTCGTCGCGCACNARNCKCAT | ||
ACR3(1)_F | GAAGGAGATGTCCTTCCTCGAYMGNTGGYT | ACR3(1) (964) | This study |
ACR3(1)_R | GGACTTCGATCAGCGGNCCNACNAC |