Table 4 Central composite design of variables (in coded levels), with extracellular chitinase activity as response
Run | Level | Chitinase activity (U/ml) | ||||
|---|---|---|---|---|---|---|
A | B | C | D | Observed | Predicted | |
1 | −1 | −1 | −1 | −1 | 7.421 | 6.368 |
2 | 1 | −1 | −1 | −1 | 9.226 | 7.061 |
3 | −1 | 1 | −1 | −1 | 9.358 | 8.204 |
4 | 1 | 1 | −1 | −1 | 9.785 | 8.758 |
5 | −1 | −1 | 1 | −1 | 6.987 | 5.908 |
6 | 1 | −1 | 1 | −1 | 6.206 | 5.837 |
7 | −1 | 1 | 1 | −1 | 9.752 | 7.944 |
8 | 1 | 1 | 1 | −1 | 9.456 | 7.732 |
9 | −1 | −1 | −1 | 1 | 1.855 | 1.426 |
10 | 1 | −1 | −1 | 1 | 1.786 | 1.884 |
11 | −1 | 1 | −1 | 1 | 6.051 | 4.710 |
12 | 1 | 1 | −1 | 1 | 6.104 | 5.029 |
13 | −1 | −1 | 1 | 1 | 1.487 | 0.804 |
14 | 1 | −1 | 1 | 1 | 1.497 | 0.497 |
15 | −1 | 1 | 1 | 1 | 4.275 | 4.287 |
16 | 1 | 1 | 1 | 1 | 4.498 | 3.841 |
17 | −2 | 0 | 0 | 0 | 1.471 | 3.306 |
18 | 2 | 0 | 0 | 0 | 1.527 | 3.553 |
19 | 0 | −2 | 0 | 0 | 1.763 | 3.171 |
20 | 0 | 2 | 0 | 0 | 5.897 | 8.351 |
21 | 0 | 0 | −2 | 0 | 2.948 | 5.089 |
22 | 0 | 0 | 2 | 0 | 1.720 | 3.442 |
23 | 0 | 0 | 0 | −2 | 7.775 | 11.033 |
24 | 0 | 0 | 0 | 2 | 1.596 | 2.200 |
25 | 0 | 0 | 0 | 0 | 1.546 | 1.557 |
26 | 0 | 0 | 0 | 0 | 1.563 | 1.557 |
27 | 0 | 0 | 0 | 0 | 1.582 | 1.557 |
28 | 0 | 0 | 0 | 0 | 1.537 | 1.557 |