From: Biodegradation optimization and metabolite elucidation of Reactive Red 120 by four different Aspergillus species isolated from soil contaminated with industrial effluent
Variables
Range of variables and level
Step change value (ΔZi)
−2
−1
0
+1
+2
Glucose (g/L)
2
6
10
14
16
4
Ammonium sulfate (g/L)
0.5
1
1.5
2.5
pH
5.0
5.5
6.0
6.5
7.0
Temperature (°C)
20
25
30
35
40
5