Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

Table 1 Sequences of primers used in the transcriptional analysis by RT-qPCR, with respective sizes of amplification fragments and annealing temperatures

Gene	Nucleotide sequence	Amplicon size (bp)	Annealing temperature (°C)
actA	5′ AGAAATCATCCGGGAAACAG 3′	147	58.98
actA	5′ CCTCTCCCGTTCAACTCTTC 3′		58.87
agrA	5′ CGGGTACTTGCCTGTATGAA 3′	149	58.65
agrA	5′ TGAATAGTTGGCGCTGTCTC 3′		59.03
crp	5′ ATTCACAGTTTGCGAATGCT 3’	117	58.86
crp	5′ TTTGCAAATCAACATCACGA 3’		59.02
degU	5′ GGCGCGTATATTCATCCAC 3’	150	58.96
degU	5′ TACCTCGCACTCTCTATGCG 3’		59.20
fri	5′ GGCGAACAAATGGATGAAGT 3’	108	59.94
fri	5′ ATAAGGCGCTTCTTCTACGC 3’		58.77
fur	5′ TTTAGCGCCTTCTTGTCTCA 3’	114	58.80
fur	5′ GGCCTTGCAACCGTTTATAG 3’		59.61
hly	5′ AGCTCATTTCACATCGTCCA 3’	124	59.24
hly	5′ TGGTAAGTTCCGGTCATCAA 3’		58.97
iscR	5′ ATCGGACCTCTTCGTAATGC 3’	106	59.15
iscR	5′ CGTATGATATCACCCGCAGT 3’		58.48
malR	5′ GAATCGTCTGGACCGGTAAT 3’	110	58.86
malR	5′ AACGTGAGCCAAGTCCTTCT 3’		58.94
prfA	5′ GGAAGCTTGGCTCTATTTGC 3’	145	59.07
prfA	5′ ACAGCTGAGCTATGTGCGAT 3’		58.65
sigB	5′ TGGTGTCACGGAAGAAGAAG 3’	135	58.85
sigB	5′ TCCGTACCACCAACAACATC 3’		59.27
sod	5′ CCACCATTTGGGCTAAGAAT 3’	94	58.90
sod	5′ GCGTTCCTGAAGATATTCGC 3’		59.81

ISSN: 1869-2044