Table 1 Strains, plasmids, and primers used in this study

 BCTRP

E. coli ΔtrpR Δtna, aroG ^fbr trpE ^fbr DCBA Am^R

Lab collection

 BCTRPP

Derived from BCTRP, but Δpta

This study

 BCTRPG

Derived from BCTRP, but ΔptsG

This study

 BCTRPPG

Derived from BCTRP, but Δpta and ΔptsG

This study

 pKD46

Am^R, λ Red-expressing vector

Datsenko and Wanner (2000)

 pKD3

Cm^R, template vector

Datsenko and Wanner (2000)

 pCP20

Am^R, Cm^R, FLP-expressing vector

Datsenko and Wanner (2000)

  pta-P1^a

5′-GCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCTTGAGCGATTGTGTAGGCTGGAG-3′^b

This study

  pta-P2^a

5′-TAGTGATTATTTCCGGTTCAGATATCCGCAGCGCAAAGCTGCGGATGATGACGAGATAACGGCTGACATGGGAATTAGC-3′^b

This study

  pta-P3

5′-GTTTCGGCAAATCTGGTTTCATC-3′

This study

  pta-P4

5′-TGGTAAGTATGCAAAGTGGGATGG-3′

This study

  ptsG-P1^a

5′-ATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCTTGAGCGATTGTGTAGGCTGGAG-3′^b

This study

  ptsG-P2^a

5′-TTAGTGGTTACGGATGTACTCATCCATCTCGGTTTTCAGGTTATCGGATTTAGTACTAACGGCTGACATGGGAATTAGC-3′^b

This study

  ptsG-P3

5′-AGTTGAAACGTGATAGCCGTC-3′

This study

  ptsG-P4

5′-AACGTGGAAGGTTCTATCGTCTAC-3′

This study