Statistically optimized production and characterization of vanillin from creosol using newly isolated Klebsiella pneumoniae P27

Table 1 Plackett-Burman experimental design of six variables for evaluating factors influencing vanillin production

Run no.	Yeast extract (g/L)	NH₄NO₃ (g/L)	K₂HPO₄ (mg/L)	MgSO₄ (mg/L)	Creosol (mL/L)	Inoculum size (mL/L)	Vanillin production (mg/L)
Run no.	Yeast extract (g/L)	NH₄NO₃ (g/L)	K₂HPO₄ (mg/L)	MgSO₄ (mg/L)	Creosol (mL/L)	Inoculum size (mL/L)	Experimental	Predicted
1	4	2	300	200	0.7	30	10.96	12.05
2	6	1.5	200	400	0.5	20	18.96	14.88
3	4	2	300	600	0.3	10	15.55	15.71
4	4	1	100	200	0.3	10	12.11	11.52
5	8	1	100	200	0.7	10	17.33	16.27
6	8	2	100	200	0.3	30	23.25	24.11
7	8	2	100	600	0.7	30	22.29	20.46
8	4	2	100	600	0.7	10	11.55	12.05
9	4	1	300	200	0.7	30	5.62	7.87
10	8	1	300	600	0.3	30	16.14	19.93
11	8	2	300	200	0.3	10	24.92	24.11
12	8	1	300	600	0.7	10	17.25	16.27
13	4	1	100	600	0.3	30	14.96	11.52

ISSN: 1869-2044