Statistically optimized production and characterization of vanillin from creosol using newly isolated Klebsiella pneumoniae P27

Table 2 Experimental design and results of central composite design (CCD) for vanillin production

Run no.	Creosol (mL/L)	Yeast extract (g/L)	NH₄NO₃ (g/L)	Vanillin production (mg/L)
Run no.	Creosol (mL/L)	Yeast extract (g/L)	NH₄NO₃ (g/L)	Experimental	Predicted
1	0.1	4	1	21.18	19.57
2	0.3	4	1	16.14	15.77
3	0.1	12	1	11.18	11.28
4	0.3	12	1	11.08	9.56
5	0.1	4	3	7.14	7.60
6	0.3	4	3	18.22	17.94
7	0.1	12	3	9.70	9.89
8	0.3	12	3	20	21.43
9	0.03	8	2	13.85	13.76
10	0.37	8	2	19.92	20.27
11	0.2	1.27	2	7.14	7.60
12	0.2	14.72	2	3.77	3.57
13	0.2	8	0.31	20.11	22.05
14	0.2	8	3.68	24.40	27.43
15	0.2	8	2	24.40	22.72
16	0.2	8	2	25	27.43
17	0.2	8	2	28.70	27.43
18	0.2	8	2	28.70	27.43
19	0.2	8	2	30.81	27.43
20	0.2	8	2	27	27.43

ISSN: 1869-2044