Table 2 Results of sugar fermentation tests vis-à-vis coding sequences for enzymes involved in utilizing various sugars as the carbon source, present in P. acidilactici NRCC1
S. no. | Sugar | API 20A result | Roll tubes | CDS for enzyme(s) involved in carbon source utilization |
|---|---|---|---|---|
1. | Arabinose | + | Not included | L-ribulose-5-phosphate 4-epimerase, ribulokinase, L-arabinose isomerase |
2. | Galactose | Not included | + | 6-Phosphofructokinase, galactosamine-6-phosphate isomerase |
3. | Glucose | + | Not included | Glucose-6-phosphate 1-dehydrogenase, glucose 1 dehydrogenase |
4. | Glycerol | + | Not included | 2-Hydroxy-3-oxopropionate reductase, pyruvate kinase, glycerate kinase |
5. | Esculin | + | Beta-glucosidase | |
6. | Mannose | + | Not included | Alpha-mannosidase, phosphomannomutase, mannose-6-phosphate isomerase |
7. | Rhamnose | + | Not included | Rhamnulose-1-phosphate aldolase, rhamnulokinase, L-rhamnose isomerase, alpha-L-rhamnosidase |
8. | Trehalose | + | Not included | Trehalose-6-phosphate hydrolase, trehalose-specific IIB component |
9. | Xylose | + | Not included | Xylulose-5-phosphate phosphoketolase |