Dairy propionibacteria as direct-fed microbials: in vitro effect on acid metabolism of Streptococcus bovis and Megasphaera elsdenii

Table 1 Sample sets prepared for analysis of the effects of inoculation of different bacterial combinations on acid metabolism in the rumen content

Group	Inoculants^a
Control	2 ml saline
SB	1 ml Streptococcus bovis + 1 ml saline
Two-strain-inoculation
PJ702 + SB	1 ml S.bovis + 1 ml Propionibacterium jensenii 702
PA25562 + SB	1 ml S.bovis + 1 ml Propionibacterium acidopropionici ATCC 25562
PA341 + SB	1 ml S.bovis + 1 ml P. acidopropionici 341
PF2206 + SB	1 ml S.bovis + 1 ml P. freudenreichii CSCC 2206
PF2207 + SB	1 ml S.bovis + 1 ml P. freudenreichii CSCC 2207
ME+SB	1 ml S.bovis + 1 ml Megashaera elsdenii
Three-strain-inoculation
PJ702 + ME+SB	1 ml S.bovis + 1 ml P. jensenii 702 + 1 ml M.elsdenii

^aInoculate environment for each group listed above was 300 g rumen sample with 1% glucose (w/w)

ISSN: 1869-2044