Table 1 Culture-independent approaches used for microbial community analysis in different plant species
From: Prospecting the characteristics and significance of the phyllosphere microbiome
Host species | Method | Dominating species | References |
|---|---|---|---|
Monocotyledons | |||
 Cultivated rice | 16s rRNA, metaproteomics | Actinobacteria, Proteobacteria | Knief et al. (2012) |
Fluorescent nuclear staining technique | Sphingomonas, Acidovorax, Methylobacterium, Pseudomonas, Sinorhizobium | Niwa et al. (2011) | |
PCR-DGGE | Proteobacteria, Firmicutes, Planctomycetes, Cyanobacteria, Actinobacteria | Thapa et al. (2018) | |
 Wheat | 16S rDNA clone library, PCR-DGGE | γ-Proteobacteria | Gu et al. (2010) |
 Maize | PCR-DGGE, DNA Hybridization | Sphingomonas, Acinetobacter | Kadivar and Stapleton (2003) |
 Barley | PCR, T-RFLP, RISA | γ-Proteobacteria | Gravouil (2012) |
 Seagrasses | 16S rRNA-DGGE analysis | Cytophaga, Flavobacteria, Bacteroides | Uku et al. (2007) |
Dicotyledons | |||
 Tropical tree species | 16S rRNA pyrosequencing | Alphaproteobacteria, Acidobacteria, Gammaproteobacteria | Kim et al. (2012) |
 Soybean, clover | 16S rRNA-DGGE and metaproteomics | Alphaproteobacteria, Sphingomonas sp., Methylobacterium | Delmotte et al. (2009) |
 Apple | Culture dependent and 16S rRNA analysis | Sphingomonadales, Actinomycetales, Rhizobiales, Pseudomonadales, Burkholderiales | Yashiro et al. (2011) |
 3 plant species (Amygdalus communis, Citrus paradisi, Nicotiana glauca) | Culture dependent and DGGE | Bacilli, Actinobacteria | Izhaki et al. (2013) |
 Lettuce | Pyrosequencing and 16S rRNA analysis | Proteobacteria, Firmicutes, Actinobacteria | Williams et al. (2013) |
 Tomato | 16S rRNA analysis and DGGE | Bacillus, Pseudomonadales, Curtobacterium, Sphingomonas | Enya et al. (2007) |
 Arabidopsis | 16S rRNA analysis and Genome wide association studies (GWAS) | Proteobacteria, Bacteroidetes, Actinobacteria | Horton et al. (2014) |