Table 2 Characteristics distinguishing tested isolates
From: Diversity and symbiotic divergence of endophytic and non-endophytic rhizobia of Medicago sativa
Distinctive characteristics | Groupsa and their reactions | |||||
|---|---|---|---|---|---|---|
A (10)b | B (2) | C (8) | D (1) | E (9) | F (2) | |
Application of sole carbon and nitrogen source (1%) | ||||||
 D-Fructose | +c (9)d | + | +(7) | + | + | + |
 L-tryptophan | +(6) | +(1) | +(4) | – | +(2) | – |
 Glycine | + | + | +(5) | – | +(1) | – |
 Arginine | + | + | + | – | +(4) | + |
 L-Histidine | + | + | + | – | +(3) | + |
 Phenylalanine | + | + | +(7) | – | +(2) | – |
Tolerance to dyes (1%) | ||||||
 Bromothymol blue | + | + | + | + | + | +(1) |
 Methyl red | +(8) | +(1) | + | + | + | +(1) |
 Bromphenol blue | +(9) | + | +(6) | + | + | + |
 Methylene blue | + | + | +(4) | – | +(6) | – |
 Malachite green | + | + | + | + | +(7) | + |
Tolerance to NaCl (%), pH, temperature (°C) | ||||||
 NaCl (6%) | +(5) | + | +(2) | – | +(1) | + |
 pH 11.0 | + | + | +(5) | + | + | – |
 8 °C | +(9) | + | +(1) | – | +(1) | – |
 37 °C | + | + | + | – | + | + |
 40 °C | + | +(1) | + | – | + | + |
Tolerance to antibiotics | ||||||
 Erythromycin [b]e | + | + | + | – | +(8) | – |
 Erythromycin [c] | +(9) | + | +(2) | – | +(7) | – |
 Erythromycin [d] | +(3) | + | – | – | +(1) | – |
 Chloromycetin [d] | +(9) | + | + | – | + | +(1) |
 Kanamycin [a] | +(9) | + | + | + | + | – |
 Kanamycin [b] | +(3) | – | +(1) | – | + | – |
Tolerance to antibiotics | ||||||
 Kanamycin [c] | +(4) | + | – | – | +(7) | – |
 Kanamycin [d] | +(4) | +(1) | – | – | +(8) | – |
 Ampicillin [b] | + | + | +(7) | + | + | – |
 Ampicillin [c] | +(9) | + | +(4) | + | + | – |
 Ampicillin [d] | + | + | +(7) | + | + | – |
 Neomycin [a] | + | + | + | + | + | +(1) |
 Neomycin [b] | + | +(1) | +(1) | + | + | – |
 Neomycin [c] | +(9) | + | +(4) | + | + | +(1) |
 Neomycin [d] | +(7) | + | +(3) | + | + | +(1) |
 Streptomycin [a] | + | + | +(6) | + | + | + |
 Streptomycin [b] | +(6) | + | +(3) | + | +(6) | + |
 Streptomycin [c] | +(9) | + | +(7) | + | + | + |
 Streptomycin [d] | +(9) | + | +(6) | + | + | +(1) |
 Gentamycin [a] | +(9) | + | +(7) | + | + | – |
 Gentamycin [b] | +(4) | + | – | – | + | – |
 Gentamycin [c] | – | + | – | – | + | – |
 Gentamycin [d] | – | +(1) | – | – | +(6) | – |
Others | ||||||
 Amylolysis | + | – | +(3) | + | +(7) | + |
 Gelatin hydrolysis | +(8) | – | +(6) | – | +(4) | +(1) |
 Voges-Proskauer test | +(5) | – | – | – | – | – |
 Indole production | +(3) | – | +(5) | – | +(4) | – |
 BTB acid production | + | +(1) | + | + | + | – |
 Citrate utilization | + | – | +(3) | – | +(2) | – |
 3-Keto-lactose production | +(1) | – | +(2) | – | +(1) | – |
 Phosphate solubilization | + | + | +(6) | – | +(2) | – |
 Indole-3-acetic acid (IAA) production | +(6) | – | +(3) | + | +(3) | – |