Diversity and symbiotic divergence of endophytic and non-endophytic rhizobia of Medicago sativa

Annals of Microbiology

Table 4 RFLP analysis of PCR-amplified 16S rRNA and concatenated housekeeping gene groups of 18 representative strains

Representative strain	Restriction types^a of amplified 16S rRNAs digested with				16S rRNA RFLP patterns^b	16S rRNA RFLP clusters^c	Group^d concatenated
Representative strain	AluI	HaeIII	HinfI	MspI	16S rRNA RFLP patterns^b	16S rRNA RFLP clusters^c	Group^d concatenated
WLP2	A	E	a	l	I (7)	i	3
G3L3	A	F	b	m	II	i	3
LP3	A	F	b	n	III	i	3
WTT6	B	G	c	o	IV	ii	2
G3G1	D	I	e	q	VII	v	1a
G3G2	D	J	f	r	VII	iv	1a
G3L1	D	K	g	s	IX	v	1d
G3P2	D	L	h	t	X	iv	1d
G3T1	D	M	i	u	XI	v	1d
G9TT1	D	N	f	v	XII (5)	iv	1b
LT2	D	O	h	w	XIII	iv	1d
LT3	D	P	j	x	XIV	iv	1c
WG2	D	P	h	y	XV (5)	iv	1d
WL2	D	P	d	y	XVI	iv	1d
WN3	D	M	k	z	XVII	v	1d
WTT4	D	J	d	u	XVII	iv	1d
Rhizobium ipomoeae shin9-1^T	A	H	d	p	V	iii	—^e
Ensifer medicae WSM419^T	C	E	a	l	VI	i	–

^aLetters (A–J, a–z) refer to 16S rRNA RFLP pattern types of tested strains detected with each restriction enzyme
^bRoman numerals refer to 16S rRNA RFLP patterns of tested strains identified by the combined analysis of 16S rRNA restriction profiles obtained with endonucleases AluI, HaeIII, HinfI, and MspI
^cCluster refer to the results gained from Fig. 2
^dGroup based on the phylogenetic tree of concatenated sequence of atpD, glnII, and recA genes
^eReference type strains Rhizobium ipomoeae shin9-1^T and Ensifer medicae WSM419^T were not included in the concatenated analysis of atpD, glnII, and recA gene sequences

ISSN: 1869-2044