Bacterial community structure associated with the rhizosphere soils and roots of Stellera chamaejasme L. along a Tibetan elevation gradient

Table 3 Statistical analysis of 16S rRNA gene clone libraries derived from rhizosphere and root samples of S. chamaejasme, calculated at 97% sequence similarity by MOTHUR program

Dataset^a		No. of sequences	No. of OTUs^b	Relative richness (%)^c	Chao 1	H′	E	LC (%)^d	Singletons (%)^e
Rs	JD	61	45	73.77	156.51	3.62	0.95	39.34	60.66
	MZGK	64	49	76.56	276.09	3.69	0.95	32.81	67.19
	YBJ	65	48	73.85	323.61	3.70	0.95	36.92	63.08
	TGL	62	46	74.19	136.37	3.71	0.97	41.94	58.06
	NMC	64	42	65.63	125.11	3.52	0.94	50.00	50.00
R	JD	62	23	37.10	33.65	2.47	0.79	75.81	24.19
	MZGK	61	37	60.66	145.75	3.23	0.89	50.82	49.18
	YBJ	65	25	38.46	44.60	2.42	0.75	74.24	25.76
	TGL	62	14	22.58	18.50	1.66	0.63	83.87	16.13
	NMC	63	6	9.52	6.75	1.04	0.58	96.83	3.17

OTUs, operational taxonomic units; H′, Shannon’s diversity index; E, Shannon’s evenness index; LC, library coverage
^aRs, rhizosphere; R, root; JD, Jinda; MZGK, Mozhugongka; YBJ, Yangbajing; TGL, Tanggula; NMC, Namucuo
^bNo. of OTUs were determined using the MOTHUR software based on the 3% sequence difference
^cRelative richness, defined as the number of OTUs observed regarding to the number of sequences
^dLibrary coverage was calculated using the formula: [1-(singletons/individuals)] × 100
^eRelative number of singletons regarding to the OTUs occurring only once in the overall clone library

ISSN: 1869-2044