Fig. 2From: Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltansSDS-PAGE (12%, w/v) showing single protein band of size, 76Â kDa, of PsPL12a; lane 1: protein ladder (250Â kDa); lane 2: uninduced cells BL21 (DE3); lane 3: induced cells BL21 (DE3); lane 4: cell pellet after sonication; lane 5: cell-free extract; lane 6: last wash from column; lane 7: purified protein (76Â kDa)Back to article page