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Table 1 Effect of metal ions on activity of PsPL12a using heparin as substrate

From: Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans

Metal ion

Concentration (mM)

Relative activity (%)

Control

–

100

Mg2+

10

186 ± 0.7

Mn2+

10

180 ± 0.8

Ca2+

10

124 ± 0.9

Na+

50

118 ± 1.8

Co2+

10

90 ± 1.2

Ni2+

10

25 ± 3.0

EDTA

10

5.5 ± 1.3

  1. All experiments were performed in triplicate (n = 3). The values represented are mean ± standard deviation