Fig. 5
Bio-autography of P. canescens extract. Penicillium canescens extract was subjected to TLC. The chromatogram was placed on MH agar plate and molten agar seeded with the bacterial suspension of 1.5 × 108 cfu/ml was poured on the chromatogram. Post-solidification, the plates were place at 4 °C for 3 h and observed for the halo around the chromatogram (a). The antibacterial activity was confirmed by the formation of halo around the chromatogram post-spray with MTT (b)