Coproduction of menaquinone-7 and nattokinase by Bacillus subtilis using soybean curd residue as a renewable substrate combined with a dissolved oxygen control strategy

Table 1 The PB design matrix setup to the screen the primary factors for MK-7 and NK production

Run	Factors (g/L)									MK-7 production (mg/L)	Nattokinase production (U/mL)
Run	A	B	C	D	E	F	G	H	J	MK-7 production (mg/L)	Nattokinase production (U/mL)
1	100	15	15	5	20	20	10	0.8	0.8	19.62	981.10
2	100	15	5	15	20	20	20	0.3	0.3	16.83	624.63
3	100	5	5	5	10	10	10	0.3	0.3	13.49	507.07
4	150	15	15	5	20	20	10	0.3	0.3	19.45	1002.16
5	150	15	15	5	10	10	20	0.3	0.8	19.25	881.71
6	150	15	5	15	20	10	10	0.8	0.8	17.78	680.32
7	100	15	15	15	10	10	10	0.8	0.3	16.01	714.83
8	150	5	5	15	10	20	10	0.3	0.8	17.37	651.32
9	100	5	15	15	20	10	20	0.3	0.8	16.73	809.72
10	150	5	15	15	10	20	20	0.8	0.3	21.15	978.73
11	100	15	5	5	10	20	20	0.8	0.8	19.60	699.38
12	150	5	5	5	20	10	20	0.8	0.3	17.53	816.44

ISSN: 1869-2044