Table 1 Effects of monochlorobenzene concentrations and cosubstrates on degradation rates and biofilm formation of B. subtilis DKT cultured in media for 24Â h
Monochlorobenzene (mM) | Cosubstrates* | Degradation (%)** | Freely suspended cells (× 107 CFU/ml)** | Biofilm formation (× 107 CFU/slide)** |
|---|---|---|---|---|
0.1 | None | 77.2 ± 7.0d | 5.7 ± 0.5a | 10.4 ± 0.9a |
Glucose | 97.4 ± 1.7f | 10.1 ± 0.6c | 21.7 ± 2.0bc | |
Succinate | 88.7 ± 5.8ef | 10.5 ± 0.7c | 23.9 ± 2.3c | |
Citrate | 90.5 ± 5.4ef | 10.7 ± 1.1c | 26.1 ± 1.9c | |
Yeast extract | 94.4 ± 3.8ef | 9.8 ± 0.9c | 24.2 ± 2.1c | |
0.3 | None | 45.1 ± 14.3b | 6.1 ± 0.4ab | 14.3 ± 1.0ab |
Glucose | 86.4 ± 8.8e | 27.7 ± 2.4d | 60.0 ± 6.1d | |
Succinate | 76.9 ± 9.3d | 30.1 ± 4.0d | 64.2 ± 10.7de | |
Citrate | 71.0 ± 9.0d | 29.4 ± 2.5d | 68.7 ± 6.3e | |
Yeast extract | 74.8 ± 9.3d | 27.8 ± 2.6d | 63.0 ± 7.5de | |
0.5 | None | 12.9 ± 3.2a | 5.2 ± 0.6a | 9.2 ± 1.1a |
Glucose | 50.4 ± 12.6c | 8.8 ± 0.9bc | 20.1 ± 1.1bc | |
Succinate | 41.5 ± 12.6b | 9.4 ± 0.5c | 22.9 ± 2.0c | |
Citrate | 38.9 ± 11.7b | 9.1 ± 0.6c | 22.4 ± 1.2c | |
Yeast extract | 40.3 ± 6.3b | 8.8 ± 0.6bc | 21.7 ± 2.3bc |