Degradation of chlorotoluenes and chlorobenzenes by the dual-species biofilm of Comamonas testosteroni strain KT5 and Bacillus subtilis strain DKT

Table 2 Activities of enzymes extracted from B. subtilis DKT growing on benzene, chlorinated benzenes and yeast extract

Enzyme	Assay substrates	Specific enzyme activities (μmol/min/mg of protein) extracted from bacteria grew on substrates and yeast extract
Enzyme	Assay substrates	Benzene	Chlorobenzene	1,2-dichlorobenzene	Yeast extract
Diol dehydrogenase	Benzene dihydrodiol	0.307 ± 0.045	0.261 ± 0.034	0.275 ± 0.032	0.026 ± 0.015
Diol dehydrogenase	1,2-dichlorobenzene dihydrodiol	0.205 ± 0.026	0.178 ± 0.017	0.187 ± 0.015	0.017 ± 0.008
Catechol 1,2-dioxygenase	Catechol	0.242 ± 0.027	0.186 ± 0.022	0.207 ± 0.031	0.035 ± 0.012
	3-Chlorocatechol	0.106 ± 0.011	0.184 ± 0.031	0.177 ± 0.027	0.026 ± 0.011
	4-Chlorocatechol	0.082 ± 0.012	0.165 ± 0.020	0.148 ± 0.011	0.011 ± 0.005
Catechol 2,3-dioxygenase	Catechol	< 0.001	< 0.001	< 0.001	0

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