Fig. 1

Growth curves of Staphylococcus aureus grown at 37 °C for 48 h, measured at the optic density (OD) of 600 nm under different benzalkonium chloride concentrations. The concentration at which there was a linear growth inhibition was considered the MIC. The OD at 600 nm in Mueller-Hinton Broth without bacterial inoculum was measured to ensure the sterility of the growth medium (Only MHB)