Fig. 1From: Inulinase hyperproduction by Kluyveromyces marxianus through codon optimization, selection of the promoter, and high-cell-density fermentation for efficient inulin hydrolysisThe linear DNA fragments (A, B, C, and D) which were used to transform the yeast strains. (A) The promoter (GPTPS1 prom) of the TPS1 gene from G. pullulans 17-1 which was the psychrotolerant yeast isolated from a sea sediment in Antarctica + the native INU1 gene from K. marxianus KM-0. (B) The promoter (W0TPS1 prom) of the TPS1 gene from Saccharomyces sp. W0 which was the high ethanol producing yeast + the native INU1 gene from K. marxianus KM-0. (C) The promoter (KMTPS1 prom) of the TPS1 gene from K. marxianus KM-0 + the native INU1 gene from K. marxianus KM-0. (D) The promoter (KMTPS1 prom) of the TPS1 gene from K. marxianus KM-0 + the optimized INU1N gene obtained in this study. term, terminatorBack to article page