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Fig. 1 | Annals of Microbiology

Fig. 1

From: Inulinase hyperproduction by Kluyveromyces marxianus through codon optimization, selection of the promoter, and high-cell-density fermentation for efficient inulin hydrolysis

Fig. 1

The linear DNA fragments (A, B, C, and D) which were used to transform the yeast strains. (A) The promoter (GPTPS1 prom) of the TPS1 gene from G. pullulans 17-1 which was the psychrotolerant yeast isolated from a sea sediment in Antarctica + the native INU1 gene from K. marxianus KM-0. (B) The promoter (W0TPS1 prom) of the TPS1 gene from Saccharomyces sp. W0 which was the high ethanol producing yeast + the native INU1 gene from K. marxianus KM-0. (C) The promoter (KMTPS1 prom) of the TPS1 gene from K. marxianus KM-0 + the native INU1 gene from K. marxianus KM-0. (D) The promoter (KMTPS1 prom) of the TPS1 gene from K. marxianus KM-0 + the optimized INU1N gene obtained in this study. term, terminator

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