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Fig. 3 | Annals of Microbiology

Fig. 3

From: Inulinase hyperproduction by Kluyveromyces marxianus through codon optimization, selection of the promoter, and high-cell-density fermentation for efficient inulin hydrolysis

Fig. 3

The specific inulinase activities of the different mutants: the strains KM-P29 and KM-P49 were the mutants carrying the promoter of the TPS1 gene from the psychrotolerant yeast G. pullulans 17-1; the strains KM-W1 and KM-W104 were the mutants carrying the promoter of the TPS1 gene from the high-ethanol-producing yeast Saccharomyces sp. W0; the strains KM-K20 and KM-K31 were the mutants carrying the promoter of the TPS1 gene from K. marxianus KM-0. The strains KM-KN16 and KM-KN32 were the mutants carrying the optimized INU1N gene directed by the TPS1 promoter from K. marxianus KM-0. Data are given as mean ± SD, n = 3. *P < 0.05, **P < 0.01: significant difference compared with the KM-P49 group; #P < 0.05, ##P < 0.01: significant difference compared with the KM-W1 group. U/g means inulinase activity per gram of cell dry weight

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