Table 1 Selected barcoding markers and oligos for qPCR amplification of eukaryotic algae, bacteria, and fungi from subaerial biofilms
Marker | Oligo name | Sequence | Length |
|---|---|---|---|
Tufa | Tufa_F | 5′-GCTGCTCAAATGGATGGTGC-3′ | 23 bp |
Tufa_R | 5′-TCATATTTATCTAAAGTTTCACG-3′ | 20 bp | |
Rbcl | rbcl_F | 5′-TTYATGCGTTGGAGAGAYCG-3 | 20 bp |
rbcl_R | 3′-GTGCATAGCWCGGTGAATRTG-3 | 21 bp | |
ITS | ITS_F | 5′-CTTTCAACAACGATCTCTTG-3 | 21 bp |
ITS_R | 5′-TTCAAAGATTCGATGATTCAC-3 | 21 bp | |
16S | 16s_F | 5′-AGGATGCAAGCGTTATCCGG-3′ | 20 bp |
16s_R | 5′-AATCCCATTCGCTCCCCTAG-3′ | 20 bp |