Table 2 List of the novel-designed fluorescent internal probes
Maker | Oligo name | Sequence | Length |
|---|---|---|---|
Tufa | Tufa probe | (JOE)5′-YTTAAATA[+A][+A][+G][+A]AGAYCAAGT0–3′(BHQ-1) | 21 bp |
ITS1-ITS2 | ITS-1Z probe | (FAM)5′-TAG[+C]AAA[+G]T[+G][+C][+G]AT[+A]A[+C]TAG-3′(BHQ-1) | 20 bp |
ITS1-ITS2 | ITS-I2A probe | (FAM)5′-CAGCG[+A][+A][+A][+T][+G][+C][+G][+A]TAAGTAA-3′(BHQ-1) | 20 bp |
16S | 16S-eubat probe | (TAMRA)5′-GTGTAGCG[+G]T[+G]AAATGCGTAG-3′(BHQ-2) | 21 bp |