Table 2 List of the novel-designed fluorescent internal probes. Fluorochromes and quenchers were chosen to be compatible in a multiplex reaction. Letters in square brackets symbolize LNA nucleotides
Marker | Oligo name | Sequence | Length |
|---|---|---|---|
TufA | TufA probe | (JOE) 5′-YTTAAAYA[+A][+A][+G][+A]AGAYCAAGT-3′ (BHQ-1) | 21 bp |
ITS1-ITS2 | ITS-1Z probe | (FAM) 5′-TAG[+C]AAA[+G]T[+G][+C][+G]AT[+A]A[+C]TAG-3′ (BHQ-1) | 20 bp |
ITS1–1752 | ITS-2A probe | (FAM) 5′-CAGCG[+A][+A][+A][+T][+G][+C][+G][+A]TAAGTAA-3′ (BHQ-1) | 20 bp |
16S | 16S-eubat probe | (TAMRA) 5′-GTGTAGCG[+G]T[+G]AAATGCGTAG-3′ (BHQ-2) | 21 bp |