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Table 3 The effects of different protease inhibitors on BL312 MCE

From: Fermentation conditions of serine/alkaline milk-clotting enzyme production by newly isolated Bacillus licheniformis BL312

Inhibitor

Concentration (mM)

Relative MCA (%)

Inhibition rate (%)

None

–

100

0

Pepstatin A

0.01

95.7 ± 1.5

4.3

 

0.02

96.9 ± 1.0

3.1

EDTA

1

97.1 ± 2.1

2.9

 

5

83.0 ± 2.3

17.0

 

10

70.6 ± 3.2

29.4

 

25

59.6 ± 2.1

40.4

PMSF

1

9.7 ± 1.7

90.3

 

2

7.1 ± 2.2

92.9

DTT

1

98.6 ± 1.4

1.4

  1. The data are represented as mean ± SD (n = 3)
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Annals of Microbiology

ISSN: 1869-2044

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