Fig. 1
GAD system in Lb. brevis and genes expression in response to 30 mg/ml of monosodium glutamate. (A) The gad operon genes and gadB gene in Lb. brevis; (B) RT-qPCR has been used to assess the transcription level of the gad in DSM 32386 and DSM 20054 as described in ‘Material and methods’ section. Comparative critical threshold method (2−ΔΔCt) was used to calculate the relative gene expression with respect to time 0. Data are presented as mean ± sd, N = 3. *p < 0.05, **p < 0.01, paired t test, DSM 32386 relative expression vs DSM 20054 relative expression