Table 4 Gene specific primers and condition for PCR reactions
Antibiotic | Antibiotic resistance cassette | Primers 5′-3′ | Annealing temperatures (°C) | Size (bp) | References |
|---|---|---|---|---|---|
Ampicillin | mecA | CGTSTTTAACTAAGTATSGY GGGATCATAGCGTCATTATTC | 58 | 1429 | Guo et al. (2017) |
bla | TAGGTTCAGATTGGCCCTTAG CATARTTCCGATAATASMGCC | 51 | 297 | Guo et al. (2017) | |
claZ | GCATGRTAACCATCACAWAC ACTTCAACACCTGCTGCTTTC | 58 | 240 | Guo et al. (2017) | |
Erythromycin | erm(A) | AAGCGGTAAACCCCTCTGA TTCGCAAATCCCTTCTCAAC | 55 | 190 | Nawaz et al. (2011) |
erm(B) | GAAAAGRTACTCAACCAAATA AGTAACGGTACTTAAATTGTTTAC | 52 | 642 | Nawaz et al. (2011) | |
Gentamycin | aac | CCAAGAGCAATAAGGGCATA CACTATCATAACCACTACCG | 60 | 220 | Nawaz et al. (2011) |
Clindamycin | lun(A) | GCTAATATTGTTTAAATCGTCAAT GGTGGCTGGGGGGTAGATGTATTAACTGG | 55 | 323 | Guo et al. (2017) |
lun(B) | GCTTCTTTTGAAATACATGGTATTTTTCGATC CCTACCTATTGTTTGTGGAA | 54 | 925 | Guo et al. (2017) | |
Tetracycline | tetM1 | GCTTGATCCCCAGTAAGTCA GGTGAACATCATAGACACGC | 55 | 401 | Guo et al. (2017) |
tetL1 | GTMGTTGCGCGCTATATTCC GTGAAMGRWAGCCCACCTAA | 55 | 696 | Fukao et al. (2009) | |
tet(W) | GAGAGCCTGCTATATGCCAGC GGGCGTATCCACAATGTTAAC | 64 | 168 | Fukao et al. (2009) | |
tet(S) | ATCAAGATATTAAGGAC TTCTCTATGTGGTAATC | 56 | 573 | Nawaz et al. (2011) | |
tet(L) | CATTTGGTCTTATTGGATCG ATTACACTTCCGATTTCGG | 50 | 456 | Nawaz et al. (2011) | |
tet(K) | TTAGGTGAAGGGTTAGGTCC GCAAACTCATTCCAGAAGCA | 55 | 697 | Nawaz et al. (2011) | |
tet(O) | AACTTAGGCATTCTGGCTCAC TCCCACTGTTCCATATCGTCA | 52 | 515 | Nawaz et al. (2011) | |
tet(Q) | AGAATCTGCTGTTTGCCAGTG CGGAGTGTCAATGATATTGCA | 56 | 169 | Nawaz et al. (2011) | |
tet(M) | GTTAAATAGTGTTCTTGGAG CTAAGATATGGCTCTAACAA | 55 | 576 | Nawaz et al. (2011) |