Expression of d-psicose-3-epimerase from Clostridium bolteae and Dorea sp. and whole-cell production of d-psicose in Bacillus subtilis

Table 1 Strains and plasmids used in this study

Strains and plasmids	Description	Source
Strains
Escherichia coli JM109	Cloning host	Sangon
Bacillus subtilis WB800	Expression host	Lab stock
WB800/pSTOP1622	WB800 harbouring pSTOP1622	This work
WB800/pSTOP1622-Cb-DPEase	WB800 harbouring pSTOP1622-Cb-DPEase	This work
WB800/pSTOP1622-P43-Cb-DPEase	WB800 harbouring pSTOP1622-P43-Cb-DPEase	This work
WB800/pSTOP1622-Ds-DPEase	WB800 harbouring pSTOP1622-Ds-DPEase	This work
WB800/pSTOP1622-P43-Ds-DPEase	WB800 harbouring pSTOP1622-P43-Ds-DPEase	This work
Plasmids
T-vector-pMD19 (simple)		TaKaRa
PUC-Cb-DPEase	Amp^r; lacZ	Sangon
PUC-Ds-DPEase	Amp^r; lacZ	Sangon
pSTOP1622	Amp^r; Tet^r, E. coil/B. subtilis shuttle plasmid, PxylA promoter	Lab stock
pSTOP1622-Cb-DPEase	Amp^r; Tet^r, pSTOP1622/Cb	This work
pSTOP1622-P43-Cb-DPEase	Amp^r; Tet^r, pSTOP1622/ P43-Cb	This work
pSTOP1622-Ds-DPEase	Amp^r; Tet^r, pSTOP1622/Ds	This work
pSTOP1622-P43-Ds-DPEase	Amp^r; Tet^r, pSTOP1622/P43-Ds	This work

ISSN: 1869-2044