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Fig. 5 | Annals of Microbiology

Fig. 5

From: Antifungal activity of volatile compounds produced by endophytic Bacillus subtilis DZSY21 against Curvularia lunata

Fig. 5

Effects of VOCs produced by B. subtilis DZSY21 on intracellular reactive oxygen species (ROS) generation in C. lunata conidia. a Detection of ROS was based on 2,7-dichlorodihydrofluorescein diacetate staining following 3 days of exposure to 5 μL 2-MBA, 17.5 μL 2-HA, or 30 μL IA below their minimum inhibitory amounts, or control (50 μL dimethyl sulfoxide [DMSO]). Red scale bars represent 20 μm. b Fluorescence intensities of conidia analyzed using the ImageJ software. Values represent the means of 30 randomly selected conidia. *A significant difference compared with the control (P < 0.05). Error bars represent the standard deviation. The assay was repeated three times

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Annals of Microbiology

ISSN: 1869-2044

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