Production, purification, and characterization of cellulase from Acinetobacter junii GAC 16.2, a novel cellulolytic gut isolate of Gryllotalpa africana, and its effects on cotton fiber and sawdust

Table 3 Summary of cellulase purification of GAC 16.2

Sample	Volume [ml]	Total protein [mg]	Protein Conc. [mg/ml]	Total activity [Units]	Yield [%]	Specific activity [Unit/mg]	Purification [fold]
Cell-free supernatant	100	261.6	2.61	112.38	100	0.43	1
Dialyzed fraction	21	26.37	1.25	68.28	60.75	2.59	6.02
Column chromatographic fraction	9.5	10.14	1.06	40.05	35.64	3.95	9.2

ISSN: 1869-2044