Diversity of hydrolase-producing halophilic bacteria and evaluation of their enzymatic activities in submerged cultures

Table 1 Physical and chemical properties of the samples from the three sites

	Site 1, JZ					Site 2, M			Site 3, BM
	WS^c	WM^d	SS	S^d	M^d	W^c	S^d	M^d	W^c	S^d	M^d
Samples classification	Moderately saline	Highly saline	Extremely saline	Saline	Slightly saline	Highly saline	Saline	Slightly saline	Highly saline	Saline	Slightly saline
pH^a	7.0	7.0	NA	7.7	7.9	8.0	7.6	8.1	7.5	7.7	8.3
Conductivity (dS/m)^b	2.1	9.3	13.9	10.5	5.4	10.6	9.4	4.9	11.3	9.2	5.4
Salinity (%)	4.5	19.5	29.2	22.0	12.0	22.4	19.7	10.4	23.7	19.3	11.2
CFU/g	ND	ND	1.25.10⁵	2.9.10⁵	3.3.10⁶	ND	2.85.10⁶	2.85.10⁶	ND	2.95.10⁶	2.85.10⁶
CFU/L	1.5. 10⁶	2.2.10⁵	ND	ND	ND	2.25.10⁶	ND	ND	2.85.10⁵	ND	ND
Number of halophilic	ND	17	13	71	65	17	14	8	6	4	12
Number of hydrolases-producing bacteria	ND	12	13	64	55	15	14	8	5	2	1
Total	166					39			22

WS water of the source, WM water inside the mine, SS salt sediment, W water of the site, S soil, M Mud, ND not determined
^aMinimal/maximal for the season
^bConductivity and salinity were measured by “water quality instrument, YSI Scientific”
^cThe water is saline if EC > 0.25 dS/m
^dThe soil is saline if EC > 4.0 dS/m

ISSN: 1869-2044