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Fig. 3 | Annals of Microbiology

Fig. 3

From: Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation

Fig. 3

Prokaryotic expression and enzymatic determination of β-glucosidase from A. niger AS3.4523 in E. coli. a SDS-PAGE results of prokaryotic expression of β-glucosidase. Lanes 1, 3, 5, 7, 9, 11, 13, and 15 represent supernatant proteins; lanes 2, 4, 6, 8, 10, 12, 14, and 16 represent precipitate proteins. The recombinant β-glucosidase was marked by arrow. b pNPG assay on enzymatic activity of wild-type β-glucosidase and recombinant β-glucosidase. c Signal peptide sequence of β-glucosidase (marked by underline). d Prokaryotic expression of β-glucosidase without signal peptide using 0.3 mM IPTG inducing. Lanes 1, 3, 5, and 7 represent precipitate proteins; lanes 2, 4, 6, and 8 represent supernatant proteins. Con., no IPTG groups. The recombinant β-glucosidase was marked by arrows. e pNPG assay on enzymatic activity of wild type and prokaryotic expressed β-glucosidase without signal peptide. BL21-pET28a-ΔSP represents recombinant plasmid without bgl signal peptide. Bars represent mean ± SD (n = 3). *p < 0.05; **p < 0.01

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Annals of Microbiology

ISSN: 1869-2044

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