Fig. 3

Disruption of CYP68J5 and mutant verification. a Diagram of the strategy for CYP68J5 mutation and mutant verification. b Verification of the cyp68j5 mutant by PCR analysis. PCR products were separated on a 0.8% agarose gel. Lanes 1 and 2, with primers 13F1 and 13R1, 13F2 and 13R1, respectively, using the cyp68j5 mutant genomic DNA as a template and lanes 3 and 4, with primers 13F1 and 13R1, 13F2 and 13R1, respectively, using the recipient strain TCCC41060 genomic DNA as a template, which serves as a control. c PCR amplification of the full ORF of the cyp68j5 mutant locus with primers X and Y