Table 1 The primers of 16S rRNA and pmoA genes, and thermal cycler procedures of polymerase chain reaction

A189_b (PCR)

GGNGACTGGGACTTYTGG

N-DAMO pmoA

4 min at 94 °C, followed by 35 cycles of 60 s at 94 °C, 60 s at 53 °C and 90 s at 72 °C, and finally 10 min at 72 °C

(Ettwig et al. 2009, Wang et al. 2012)

cmo682(PCR)

AAAYCCGGCRAAGAACGA

N-DAMO pmoA

cmo182(PCR)

TCACGTTGACGCCGATCC

N-DAMO pmoA

cmo568(PCR)

GCACATACCCATCCCCATC

N-DAMO pmoA

202F(PCR)

GACCAAAGGGGGCGAGCG

NC10 phylum 16S

3 min at 95 °C, followed by 40 cycles of 60 s at 95 °C, 60 s at 63 °C and 90 s at 72 °C, and finally 5 min at 72 °C

(Luesken et al. 2011, Chen et al. 2015)

1545R(PCR)

CAKAAAGGAGGTGATCC

Bacteria 16S

qP1F((q)PCR)

GGGCTTGACATCCCACGAACCTG

N-DAMO 16S

qP2R((q)PCR)

CTCAGCGACTTCGAGTACAG

N-DAMO 16S