Diagnosing bioremediation of crude oil-contaminated soil and related geochemical processes at the field scale through microbial community and functional genes

Table 1 Primers used for qPCR and thermal programs in this study

Function	Target gene	Sequence (5′-3′) of parimer pairs	Annealing (°C)	Acmplicon size (bp)	Thermal program
Total bacteria	16S rRNA	341F: CCTACGGGAGGCAGCAG	60	200	30s at 94 °C, 40 cycles of 10 s at 94 °C, 30 s at annealing temperture, and 40 s at 72 °C
Total bacteria	16S rRNA	518R: ATTACCGCGGCTGCTGG	60	200
Anammox bacteria	Amx	Amx809f: GCCGTAAACGATGGGCACT	60	282
Anammox bacteria	Amx	Amx1066r: AACGTCTCACGACACGAGCTG	60	282
Ammonia-oxidizing archaea (AOA)	amoA (AOA)	amoAF: STAATGGTCTGGCTTAGACG	53	635
Ammonia-oxidizing archaea (AOA)	amoA (AOA)	amoAR: GCGGCCATCCATCTGTATGT	53	635
Acmmonia-oxidizing bacteria (AOB)	amoA (AOB)	amoAF: GGGGTTTCTACTGGTGGT	60	491
Acmmonia-oxidizing bacteria (AOB)	amoA (AOB)	amoAR: CCCCTCKGSAAAGCCTTCTTC	60	491
Denitrifying bacteria	nosz	nosZ1F: WCSYTGTTCMTCGACAGCCAG	63	251
Denitrifying bacteria	nosz	nosZ1R: ATGTCGATCARCTGVKCRTTYTC	63	251
Dissimilarity nitrite reducing bacteria	napA	napA3F: CCCAATGCTCGCCACTG	60	130
Dissimilarity nitrite reducing bacteria	napA	napA3R: CATGTTKGAGCCCCACAG	60	130
Alkane-degrading bacteria	alkB	alkBf :AAYACNGCNCAYGARCTNGGNCAYAA	55	550
Alkane-degrading bacteria	alkB	alkBr:GCRTGRTGRTCNGARTGNCGYTG	55	550
Alcanivorax	16S rRNA	Alcvx464F: GAGTACTTGACGTTACCTACAG	60	220
Alcanivorax	16S rRNA	Alcvx675R: ACCGGAAATTCCACCTC	60	220

ISSN: 1869-2044