Table 2 Alteration of specificities of XRs and XDHs of different yeast strains
Yeast | Mutation | Purpose | Reference |
|---|---|---|---|
XR mutants | |||
C. tenuis | K274M | NADPH to NADH preference | Petschacher and Nidetzky (2005) |
K274R | |||
S275A | |||
N276D | |||
R280H | |||
S. stipitis | K341R/N343D | NADPH to NADH preference | Dmytruk et al. (2008) |
E223A | Strict NADPH dependence | Khattab et al. (2011) | |
E223D | |||
S271A | |||
K270M | NADPH to NADH preference | Kostrzynska et al. (1998) | |
XDH mutants | |||
S. stipitis | 1208R | NAD to NADP preference | Watanabe et al. (2005) |
F209S | |||
N211R | |||
D270G | NADP preference | Metzger and Hollenberg (1995) | |
D270G/D210G | NAD to NADP preference | ||