Fig. 3

Effects of pH and temperature on the activity and stability of LacAn. a Optimum temperature of LacAn for enzyme activity was measured in 50 mM Na₂HPO₄-KH₂PO₄ at pH 7.0 with syringaldazine as a substrate. b Thermostability was investigated at 25 °C (triangle), 45 °C (circle), and 65 °C (square) by determining the enzyme activity under the same conditions as above. c Optimum pH of LacAn for enzyme activity was measured in 50 mM Na₂HPO₄-KH₂PO₄ at 75 °C with syringaldazine as a substrate. d pH stability was investigated at pH 8.5 (triangle), pH 7.5 (circle), and pH 6.5 (square) by determining the enzyme activity under the same conditions as above. Error bars represent standard deviations