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Table 3 Morphological and biochemical characterization of isolates

From: Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent

Characteristic

Bacterial isolates

ML5

ML12

MS12

Morphological features

 Color

Creamy red

White

Creamy red

 Shape

Rod

Rod

Long Rod

 Surface

Smooth

Smooth

Smooth

 Opacity

Opaque

Opaque

Opaque

 Gram staining

Positive

Positive

Positive

 Motility

Motile

Motile

Motile

 Endospore staining

Positive

Positive

Positive

Biochemical characteristic

 Sucrose hydrolysis

Positive

Positive

Positive

 Lactose hydrolysis

Negative

Negative

Positive

 Glucose hydrolysis

Positive

Positive

Positive

 Fructose hydrolysis

Positive

Positive

Negative

 Mannitol hydrolysis

Positive

Negative

Positive

 Starch hydrolysis

Positive

Positive

Positive

 Galactose hydrolysis

Positive

Positive

Positive

 Indole test

Negative

Negative

Negative

 Methyl red test

Negative

Positive

Positive

 Urease test

Negative

Negative

Positive

 Voges-Proskauer test

Positive

Negative

Positive

 Casein hydrolysis

Positive

Positive

Positive

 Citrate utilization test

Positive

Negative

Negative

 Gelatin hydrolysis

Positive

Positive

Positive

 Catalase test

Positive

Positive

Positive

 Similarity of bacteria

Bacillus sp.

Bacillus sp.

Bacillus sp.