Fig. 6

Gel electrophoresis of proteins from the purification of β-galactosidase and α-galactosidase activities from G. dipsosauri strain DD1. Panel (a) shows a nondenaturing 4–15% polyacrylamide gel. The samples were as follows: (1) a mixture of proteins from the Sephadex-G-200 columns stained for β-galactosidase activity with X-β-Gal and for α-galactosidase with X-α-Gal; (2) PM2500 molecular markers—the sizes of these proteins are indicated in the text box to the right of panel (b); (3) concentrated MacroPrep-DEAE pool containing β-galactosidase I; (4) concentrated MacroPrep-DEAE pool containing β-galactosidase II; (5) concentrated MacroPrep-DEAE pool containing α-galactosidase. Panel (b) shows the corresponding 4-15% denaturing (SDS-PAGE) gel. The samples were as follows: (6) concentrated MacroPrep-DEAE pool containing β-galactosidase I; (7) concentrated MacroPrep-DEAE pool containing β-galactosidase II; (8) concentrated MacroPrep-DEAE pool containing α-galactosidase; and 9) PM2500 molecular markers. The two gels were run separately and the proteins in lanes 2 to 9 stained with Coomassie Blue. The images of the relevant parts of the two gels were aligned so that the molecular markers matched as much as possible