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Fig. 1 | Annals of Microbiology

Fig. 1

From: Leveraging quorum sensing system for automatic coordination of Escherichia coli growth and lactic acid biosynthesis

Fig. 1

Schematic diagram of engineering quorum sensing in E. coli. A, B, C, and D indicate four recombinant E. coli strains. Each strain harbors two vectors, and the vector pluxIR is owned by all strains. Pluxi, the native promoter of luxI gene; eGFP, enhanced green fluorescent protein; AHL, acyl-homoserine lactone; LuxR-AHL complex can induce promoter luxI, and enzyme AiiA can degrade AHL. pET-Pluxi:egfp, the recombinant vector overexpressing egfp; pluxIR, the recombinant vector coexpressing LuxI and LuxR; pET-Pluxi:egfp-aiiA, the recombinant vector coexpressing egfp and aiiA; pET-Pluxi:ldhA, the vector overexpressing ldhA; pET-Pluxi:ldhA-aiiA, the vector coexpressing ldhA and aiiA; RBS, ribosome binding site; KanR, kanamycin resistance gene; CmR, chloramphenicol resistance gene

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Annals of Microbiology

ISSN: 1869-2044

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