Fig. 2

Performance of the recombinant E. coli strains harboring engineered quorum sensing circuits. A SDS-PAGE analysis of eGFP expression in recombinant E. coli strains. Lane 1, Q14; Lane 2, Q11; Lane 3, Q1; Lane 4, C02; Lane 5, C05; Lane 6, C04. Red arrowheads indicate eGFP. B Laser scanning confocal microscopic observation of eGFP expression in strain Q14 (“ON to semi-OFF”) and strain Q11 (“ON”). The upper three pictures (a, b, c) indicate eGFP expression in “ON” strain at 8 h, 20 h and 32 h, respectively; The bottom three pictures (d, e, f) indicate eGFP expression in “ON to semi-OFF” strain at 8 h, 20 h and 32 h, respectively. (C) eGFP fluorescence levels of recombinant E. coli strains. All data points are presented as mean ± s.d. from three independent experiments