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Fig. 4 | Annals of Microbiology

Fig. 4

From: Leveraging quorum sensing system for automatic coordination of Escherichia coli growth and lactic acid biosynthesis

Fig. 4

Exploring the effects of different AiiA expression level on the production of D-lactic acid by the “ON to semi-OFF” strain. A Schematic diagram of “ON to semi-OFF” strains with different AiiA expression levels. Each strain harbors two vectors, and the vector pluxIR is owned by all strains. pET-Pluxi:ldhA-aiiA (strong), the recombinant vector coexpressing ldhA and aiiA, and the latter using a strong-strength RBS. pET-Pluxi:ldhA-aiiA, the recombinant vector coexpressing ldhA and aiiA, and the latter using a medium-strength RBS, the original RBS was used for overexpressing ldhA. pET-Pluxi:ldhA-aiiA(weak), the recombinant vector coexpressing ldhA and aiiA, and the latter using a medium-strength RBS. B Comparison of aiiA transcription levels in Q17, Q15, and Q16. C Comparison of LdhA-specific activities. D Comparison of D-lactic acid titer. All data are presented as mean ± s.d. from three independent experiments

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Annals of Microbiology

ISSN: 1869-2044

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