Fig. 2
Change in size, fluorescence and culturability of Vibrio spp. strains exiting from VBNC state. Resuscitation observed by flow cytometry. A–B Cell size (FSC-A). C–D DNA quantity (green fluorescence (FL1-A) of cells stained with SYTO™ 9). E–F Green fluorescence (FL1-A) of cells stained with propidium iodide (PI). Changes detected before for cell size, DNA quantity and propidium iodide fluorescence (Fig. 1) are reverted in a different extent in the two strains. Resuscitation detected by culturability. G–H Increase in culturability, according to the CFU numbers detected plating cells on solid medium, after the incubation at 30 °C. The process of resuscitation happens exponentially. Counts (CFU/ml) are reported as Log10(CFU + 1). A, C, E and G V. campbellii BAA-1117™ strain. Black line: no incubation at 30 °C. Green line: after 17 h of incubation at 30 °C. Purple: after 21 h of incubation at 30 °C. B, D, F and H V. japonicus UU24 strain. Blue line: no incubation at 30 °C. Black line: after 1 h of incubation at 30 °C. Green line: after 24 h of incubation at 30 °C. Purple line: after 64 h of incubation at 30 °C