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Table 3 Number of copies of 16S marker gene-related enzymatic functions to nitrification and denitrification processes in the soil treatments (gene copies/g soil)

From: The effect of biochar on nitrogen availability and bacterial community in farmland

Enzyme name

Enzyme commission (EC) number

CK

BC1

BC2

BC3

BC4

Ammonia monooxygenase

1.14.99.39

274±17 b

243±28 b

219±10 c

327±23 a

337±38 a

Hydroxylamine dehydrogenase

1.7.2.6

258±26 ab

229±17 b

223±30 b

311±39 a

322±54 a

Nitrite reductase (NO-forming)

1.7.2.1

3546±131 b

3242±134 b

3409±244 b

4031±166 a

3318±128 b

Nitrate reductase

1.7.99.4

24968±480 a

25178±505 a

25469±915 a

25044±405 a

26160±886 a

Nitric-oxide reductase (cytochrome c)

1.7.2.5

2095±88 c

2121±98 bc

2322±77 a

2439±108 a

2282±74 ab

Nitrous-oxide reductase

1.7.2.4

1803±85 bc

1739±24 c

1897±82 b

2092±99 a

1899±72 b

  1. Values are mean plus standard deviation (n = 3), and data with different lowercase letters are significantly different at p < 0.05 according to Duncan’s multiple range test. Treatments CK, BC1, BC2, BC3, and BC4 had biochar dosages of 0%, 0.5%, 1%, 2%, and 4%