Table 2 Composition of Sistrom’s basal medium

K₂HPO₄

199.7 mM

or KH₂PO₄

199.8 mM

(NH₄)₂SO₄

37.8 mM

or NH₄Cl

36.55 mM

Succinic acid

338.7 mM

l-glutamic acid

5.34 mM

l-aspartic acid

3 mM

NaCl

86.2 mM

Nitrilotriacetic acid

10.4 mM

MgSO₄ . 7H₂O

12.17 mM

CaCl₂ . 2H₂O

2.27 mM

FeSO₄ . 7H₂O

0.072 mM

(NH₄)₆Mo₇0₂₄

(1% solution)

0.2 ml

Trace Elements Solution

1 ml

Vitamins Solution

1 ml