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Table 6 VA production from 5 mM vanillin in the absence or presence of 5 mM FA and nutrient supplements by P. aromaticivorans AR20-38 at 10 °C and 20 °C. Data represent mean values of three replicates

From: Bioconversion of ferulic acid and vanillin to vanillic acid by cold-adapted Paraburkholderia aromaticivorans AR20-38: impact of culture conditions

Vanillin (mM)

FA (mM)

Nutrient supplement

Temperature (°C)

Incubation time (days)

Growth (OD600)

Molar VA yield (%) from

      

5 mM vanillin

5 mM vanillin + 5 mM FA

5

0

Without

10

11

0.047

22.7 a

 

5

0

Yeast extract

10

10

1.043

90 b

 

5

0

Glucose

10

10

3.167

100.5 c

 

5

5

Without

10

11

0.048

 

15.0 a

    

32

0.052

 

31.1 b

5

5

Yeast extract

10

10

1.163

 

96.1 c

5

5

Glucose

10

10

3.357

 

95.6 c

5

0

Without

20

8

0.048

30.6 a

 

5

0

Yeast extract

20

8

0.855

101.6 b

 

5

0

Glucose

20

3

2.560

101.5 b

 

5

5

without

20

8

0.051

 

19.9 a

    

32

0.051

 

67.7 b

5

5

Yeast extract

20

8

1.130

 

97.9 c

5

5

Glucose

20

3

2.800

 

99.1 c

  1. Different letters (a-c) indicate statistically significant differences (LSD, p ≤ 0.05) between vanillin concentrations