Table 1 Tested strains and specific detect of RT-PCR
From: Quantitative detection of Vibrio alginolyticus strain XSBZ14 by a newly developed RT-PCR method
Number | Strain | Sources | CT ± SD |
|---|---|---|---|
1 | V. alginolyticus XSBZ14 | Diseased P. andrewsi, Xisha Archipelago | 11.35 ± 0.09 |
2 | V. alginolyticus ATCC17749 | ATCC | NA |
3 | V. alginolyticus HN07014 | Reared seawater, Hainan, China | NA |
4 | V. alginolyticus HN07011 | Reared seawater, Hainan, China | 32.96 ± 1.25 |
5 | V. alginolyticus HN08809 | Diseased Epinephelus coioides, Haikou | NA |
6 | V. alginolyticus HN08304 | Reared seawater, Haikou | NA |
7 | V. alginolyticus HN08307 | Reared seawater, Xincun Bay in Lingshui City | NA |
8 | V. alginolyticus HN08805 | Reared seawater, Hainan, China | NA |
9 | V. alginolyticus HN07005 | Seawater, Haikou, Hainan, China | 34.35 ± 1.78 |
10 | V. alginolyticus XSE381 | Healthy Corals, Xisha Archipelago | 35.83 ± 2.47 |
11 | V. alginolyticus HN08803 | Reared seawater, Hainan, China | NA |
12 | V. alginolyticus PBVAY30031 | Reared seawater, Hainan, China | NA |
13 | V. alginolyticus HN08203 | Reared seawater, Hainan, China | NA |
14 | V. alginolyticus HN07006 | Diseased Lutjanus argentimaculatus, Hainan, China | NA |
15 | V. alginolyticus HN08811 | Diseased Epinephelus coioides, Haikou | NA |
16 | V. alginolyticus HN08155 | Diseased Epinephelus coioides, Xincun Bay in Lingshui City | 33.06 ± 0.5 |
17 | V. alginolyticus HN07002 | Seawater, Haikou, Hainan, China | NA |
18 | V. alginolyticus TG06003 | Seawater, Hainan, China | NA |
19 | V. alginolyticus HN08306 | Seawater, Hainan, China | NA |
20 | V. alginolyticus HN07009 | Seawater, Hainan, China | NA |
21 | V. alginolyticus HN08813 | Diseased Epinephelus coioides, Haikou | NA |
22 | V. alginolyticus PBVAY39118 | Seawater, Hainan, China | NA |
23 | V. alginolyticus HN08335 | Diseased Epinephelus coioides, Xincun Bay in Lingshui | 31.96 ± 1.12 |
24 | V. alginolyticus HN08303 | Reared seawater, Hainan, China | NA |
25 | V. alginolyticus HN08801 | Reared seawater, Hainan, China | NA |
26 | V. alginolyticus HN08201 | Reared seawater, Hainan, China | 34 ± 1.8 |
27 | V. alginolyticus E167 | Seawater, Hainan, China | NA |
28 | V. alginolyticus HN08305 | Reared seawater, Hainan, China | NA |
29 | V. alginolyticus E001 | Healthy Corals, Xisha | NA |
30 | V. alginolyticus HN08156 | Pearl River Fisheries Research Institute, Guangzhou, Guangdong China | 31.39 ± 0.03 |
31 | V. alginolyticus HN07010 | Reared seawater, Hainan, China | NA |
32 | V. alginolyticus HN13001 | Diseased Epinephelus coioides, Haikou | 36.37 ± 1.23 |
33 | V. alginolyticus PBVAY40119 | Diseased Epinephelus coioides, Haikou | NA |
34 | V. alginolyticus HN08202 | Reared seawater, Hainan, China | NA |
35 | V. alginolyticus PBVAY41120 | Reared seawater, Hainan, China | NA |
36 | V. alginolyticus PBVAY38117 | Reared seawater, Hainan, China | NA |
37 | V. alginolyticus HN08806 | Reared seawater, Hainan, China | NA |
38 | V. alginolyticus PBVAY42121 | Reared seawater, Hainan, China | NA |
39 | V. alginolyticus PBVAY12023 | Reared seawater, Hainan, China | NA |
40 | V. harveyi ATCC14126 | ATCC | NA |
41 | V. harveyi WC13D121 | Diseased Lutjanus argentimaculatus, Wenchang | NA |
42 | V. harveyi HNH11011 | Diseased Epinephelus coioides, Haikou | NA |
43 | V. harveyi HNH11001 | Diseased Lutjanus sanguineus, Haikou | NA |
44 | V. harveyi WC13DH21 | Diseased Lutjanus argentimaculatus, Wenchang | NA |
45 | V. harveyi WC13H252 | Diseased Lutjanus argentimaculatus, Wenchang | NA |
46 | V. harveyi HNH11009 | Diseased Lutjanus sanguineus, Haikou | NA |
47 | V. harveyi HNH11013 | Diseased Lutjanus erythropterus, Wenchang | NA |
48 | V. harveyi WC13DH52 | Diseased Lutjanus argentimaculatus, Wenchang | 34.55 ± 0.14 |
49 | V. harveyi NS131241 | Diseased Epinephelus coioides, Sanya | NA |
50 | V. harveyi NS131751 | Diseased Epinephelus coioides, Sanya | NA |
51 | V. harveyi PBVH78461 | Diseased Epinephelus coioides, Sanya | NA |
52 | V. harveyi NS131651 | Diseased Epinephelus coioides, Sanya | NA |
53 | V. harveyi NS131451 | Diseased Epinephelus coioides, Sanya | 31.67 ± 0.35 |
54 | V. harveyi NS131631 | Diseased Epinephelus coioides, Sanya | NA |
55 | V. harveyi NS131632 | Diseased Epinephelus coioides, Sanya | NA |
56 | Vibrio owensii XSBZ03 | Diseased P. andrewsi, Xisha Archipelago | 37.34 ± 3.22 |
57 | V. owensii DSM23055 | MCCC | NA |
58 | V. owensii MCCC1A07652 | MCCC | NA |
59 | V. owensii MCCC1A06874 | MCCC | NA |
60 | V. owensii MCCC1A10877 | MCCC | NA |
61 | V. owensii MCCC1A06599 | MCCC | NA |
62 | V. owensii MCCC1A07291 | MCCC | NA |
63 | V. owensii MCCC1A06614 | MCCC | NA |
64 | V. vulnificus ATCC 27562 | ATCC | NA |
65 | Vibrio natriegens ATCC 33788 | ATCC | NA |
66 | Vibrio fluvialis ATCC 33810 | ATCC | NA |
67 | V. parahaemolyticus ATCC17802 | ATCC | NA |
68 | V. parahaemolyticus PBVPY07150 | Seawater, Hainan, China | NA |
69 | V. parahaemolyticus PBVPY06106 | Healthy corals, Xisha Archipelago | 38.11 ± 1.65 |
70 | Vibrio rotiferianus HN076 | Healthy corals, Xisha Archipelago | NA |
71 | V. coralliilyticus CAIM616 | MCCC | 32.88 ± 0.82 |
72 | Vibrio mediterranei LMG11258 | MCCC | NA |
73 | V. shilonii MCCC1A13957 | MCCC | NA |
74 | Negative control | Â | NA |