Table 1 The Plackett–Burman experimental design for eight variables
From: Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18
Trial (no.) | Variable | |||||||
|---|---|---|---|---|---|---|---|---|
A | B | C | D | E | F | G | H | |
KH2PO4 | MgSO4 | Lactose | NH4Cl | pH | Inoculum age | Inoculum size | Medium volume | |
1 | 1 | − 1 | 1 | − 1 | − 1 | − 1 | 1 | 1 |
2 | 1 | 1 | − 1 | 1 | − 1 | − 1 | − 1 | 1 |
3 | − 1 | 1 | 1 | − 1 | 1 | − 1 | − 1 | − 1 |
4 | 1 | − 1 | 1 | 1 | − 1 | 1 | − 1 | − 1 |
5 | 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | − 1 |
6 | 1 | 1 | 1 | − 1 | 1 | 1 | − 1 | 1 |
7 | − 1 | 1 | 1 | 1 | − 1 | 1 | 1 | − 1 |
8 | − 1 | − 1 | 1 | 1 | 1 | − 1 | 1 | 1 |
9 | − 1 | − 1 | − 1 | 1 | 1 | 1 | − 1 | 1 |
10 | 1 | − 1 | − 1 | − 1 | 1 | 1 | 1 | − 1 |
11 | − 1 | 1 | − 1 | − 1 | − 1 | 1 | 1 | 1 |
12 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |